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Pullulanase/Limit-dextrinase - Enzyme activity assay kit
Reference : K-PULLG6
Method for the measurement of Pullulanase/Limit Dextrinase
Countries eligible for delivery
Countries eligible for delivery:
France (except Corsica and overseas departments and territories), Algeria, French overseas departments and territories, French Polynesia, Libyan Arab Jamahiriya, Morocco, Tunisia, Egypt, France (Corsica)
Method for the measurement of Pullulanase/Limit Dextrinase
PullG6 assay for the measurement of pullulanase employs a water soluble defined substrate, namely 4,6-O-benzylidene-4-Nitrophenyl-63-α-D-Maltotriosyl-Maltotriose (BPNPG3G3), coupled with the ancillary enzymes α-glucosidase and β-Glucosidase.
Upon hydrolysis of the substrate at the 1,6-α-linkage by pullulanase or limit-dextrinase, the released 4-Nitrophenyl-β-Maltotrioside is immediately hydrolysed to glucose and 4-nitrophenol by the concerted action of the α-glucosidase and β-glucosidase enzymes in the reagent mixture.
The reaction is terminated and phenolate ions are developed by addition of dilute alkali.
he absorbance is read at 400 nm and the value obtained correlates directly with pullulanase activity.
Shipping temperature: ambient
Storage temperature:
- Short term stability: 2-8oC,
- Long term stability: see individual component labels
Assay format: spectrophotometer
Detection method: absorbance - 400
Signal response: increase
Total assay time: *10 min for pullanase preparations
*30 min for malt extracts containing limit-dextrinase
Detection limit: 18 U/ml for pullulanase preparations (50-fold dilution) and 0,01 U/g for limit dextrinase in milled malt
Reproducibility (%): ~ 3%
Stability: > 2 years under recommended storage condition
PullG6 assay for the measurement of pullulanase employs a water soluble defined substrate, namely 4,6-O-benzylidene-4-Nitrophenyl-63-α-D-Maltotriosyl-Maltotriose (BPNPG3G3), coupled with the ancillary enzymes α-glucosidase and β-Glucosidase.
Upon hydrolysis of the substrate at the 1,6-α-linkage by pullulanase or limit-dextrinase, the released 4-Nitrophenyl-β-Maltotrioside is immediately hydrolysed to glucose and 4-nitrophenol by the concerted action of the α-glucosidase and β-glucosidase enzymes in the reagent mixture.
The reaction is terminated and phenolate ions are developed by addition of dilute alkali.
he absorbance is read at 400 nm and the value obtained correlates directly with pullulanase activity.
Shipping temperature: ambient
Storage temperature:
- Short term stability: 2-8oC,
- Long term stability: see individual component labels
Assay format: spectrophotometer
Detection method: absorbance - 400
Signal response: increase
Total assay time: *10 min for pullanase preparations
*30 min for malt extracts containing limit-dextrinase
Detection limit: 18 U/ml for pullulanase preparations (50-fold dilution) and 0,01 U/g for limit dextrinase in milled malt
Reproducibility (%): ~ 3%
Stability: > 2 years under recommended storage condition
- High sensitivity
- Suitable for manual and auto-analyser formats
- No transglycosylation interference
- Very cost effective
- All reagents stable for > 1 year after preparation
- Very specific
- Simple format
- Standard included
Assay of microbial pullulanase preparations and measurement of limit-dextrinase in malt extracts
Safety data sheet
Calculator data
Validation report
Data Booklet
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Specifications
| Reference | Packaging | |
|---|---|---|
| K-PULLG6 | 100/200 tests (selon méthode/according to method) |